Regeneration for cell membrane vesicles

Hi,
Yes i think the injection time should be longer to give the trypsine some time to digest the proteins on the surface. The wash with buffer and again with Chaps and buffer. For instance, you can repeat two 10 minute injections and compare the baseline on the effectiveness of the first and second injection.

That the absolute baseline is not reached can be the debris of the vesicles but keep in mind that instruments drift sligthly over time. Best test is the surface is sufficiently clean is to inject the same vesicles again and compare the capture level.

Arnoud

I have seen the big jumps on other types of instruments. They are mainly caused by the detection algorithm that can not follow the large and fast changes in refractive index. The system assumes the wrong 'window of values' and that is the reason you find large jumps with for instance IPA.