Immobilization of a his(6x)-protein

Hello everyone!

I am fairly new to SPR and have a question about the immobilization of a purified His(6x)-protein. The size of the protein is about 35 kDa.

In the literature I found immobilization protocols using a NTA-Chip. Can I also use a CM5 oder HC200M-Chip and a covalent immobilization using EDC/NHS? Or isn't it possible because of the His-Tag?

Thanks a lot.
Best regards.

Hi Jana,

You can use the amine coupling on alomost all proteins provided that the protein has free lysines and that they are not only on the interaction site. Having a 6xHIS-tag will not impair amine coupling.

kind regards
Arnoud

Hi Arnoud,
Thanks a lot for the quick reply.
Kind regards,
Jana