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NA chip

  • mtarca
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4 years 3 months ago #1 by mtarca
NA chip was created by mtarca
Hello. It is my first time to use NA (Neutravidin chip). Does anyone have any suggestion or experience on what regeneration solution to use? I read somewhere that the NA chip is sensitive to acidic pH? Thanks.

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  • Arnoud
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4 years 3 months ago #2 by Arnoud
Replied by Arnoud on topic NA chip
Neutravidin: en.wikipedia.org/wiki/NeutrAvidin

If you want to regenerate the neutravidin-biotin interaction: this is not possible without damaging the sensor chip.

try searching for regenerable avidin

Kind regards
Arnoud

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  • mtarca
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4 years 3 months ago #3 by mtarca
Replied by mtarca on topic NA chip
Oh sorry, I should have been specific in my question. I was going to use the neutravidin chip instead of strepavidin chip. I was going to immobilize my biotinylated ligand to this NA chip, and then pass on the analyte. I was looking for a regeneration solution to knock off the analyte and leave the biotin-ligand attached to the surface for the next cycle. But since the NA chip is not compatible with acidic solutions, I could not use an acidic regent (ie. glycine-HCL 1.5), but wondering what other solutions I can use. Thanks.

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  • Arnoud
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4 years 3 months ago #4 by Arnoud
Replied by Arnoud on topic NA chip
We prefer the cocktail regeneration method ( www.sprpages.nl/kinetics/regeneration ) since this is more gentle on the ligand.
In general (Neutr)Avidin is fairly stable.

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