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measuring kinectics from cell supernants
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measuring kinectics from cell supernants?

  • nm
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4 years 3 months ago #1 by nm
I have a hybridoma supernatant with antibody and another hybridoma supernatant with an antigen, Can I measure the binding kinectics without purification?

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  • Arnoud
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4 years 3 months ago #2 by Arnoud
Replied by Arnoud on topic measuring kinectics from cell supernants?
No not like this.
You have at least capture one of the components. You can capture the antibody with a proteine A sensor chip and flow your antigen over.
Arnoud

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