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Inconsistent KD
- Biazap
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4 years 4 months ago #1
by Biazap
Inconsistent KD was created by Biazap
Hi am trying to develop a method for FcRn and am observing that KD is varying a lot within the same run. 6 sets of the same analyte was run in a single run with the same preparation to check the variability. Have observed the KD varying From 53 nM to 350 nM . Could you please suggest what has to be done?
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- Arnoud
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4 years 4 months ago #2
by Arnoud
Replied by Arnoud on topic Inconsistent KD
The numbers say that the replicates are not very well. But as told before, don't rank/judge your experiments on the KD alone!
Please give some more information. ka, kd etc, Sensorgrams, fittings...
Arnoud
Please give some more information. ka, kd etc, Sensorgrams, fittings...
Arnoud
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