Rmax fit response curve

Dear all,

I am using a system where an antibody is immobilized as Ligand on polymer brushes grafted from an Au-SPR chip, and afterwards analyte solutions are injected. The amount of ligand differs largely from chip to chip.
I would like to fit the response curves to the theoretical Rmax before analysing the binding with the open source tool Anabel.

Is it a suitable approach to just calculate the Rmax for every surface and divide the RU values of the response curves by the respective Rmax?

Help would be much appreciated

Jonas

Hi Jonas,

I presume you calculate the theoretical Rmax of a specific surface and use that number to 'normalize' the response levels. You can do that.
But for what reason? Remember that in kinetics the Rmax is more or less a scaling factor of the response level. Thus you will calculate the same kinetic parameters with native curve as you will do with the 'normalized' (except the Rmax).

Arnoud