You can calculate the Rmax of the reaction with the formulas at
www.sprpages.nl/immobilization/immobilization-procedures
.
Normally we aim for 100 RU but if you are unsure of the activity of the protein immobilize more or immobilize two or three concentrations giving a theoretical Rmax of 50, 250 and 2500 RU. Always immobilize the highest at channel 4.
If you really want to compare dufferent amino acids you should do the full kinetics.
Best way to determine the full kinetics is a six step concentration range with a 3x dilution series. You have to figure out yourself where to begin but the final concentration range should be between 0.1 and 10 xKD of the interaction.
When you run the series overnight you have 15-18 hours of measuring time!
Kind regard
Arnoud