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Negative RU - big dip after injection

  • Neeraja
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5 years 3 months ago #1 by Neeraja
Negative RU - big dip after injection was created by Neeraja
Hi, I am trying to see if my protein of interest (ligand) binds the ds RNA (analyte). My base3lines seem ok but my sample curve has a huge dip right after the injection(green curve in the picture. What does that indicate? Is there any indication of binding for this analyte sample or is it just a negative curve due to non specific binding?
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  • Arnoud
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5 years 3 months ago #2 by Arnoud
Replied by Arnoud on topic Negative RU - big dip after injection
It is difficult to say where this kind of dips come from. Generally there is a buffer mismatch or sample carry-over from the previous injection. In rare cases the refractive index near the surface is changed by a compound but in general proteins should give a positive binding curve upon interaction.

See for instance www.sprpages.nl/experiments/troubleshooting

Kind regards
Arnoud

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