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Effective surface area of SPR

  • Maedehmozneb
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5 years 11 months ago #1 by Maedehmozneb
Effective surface area of SPR was created by Maedehmozneb
Hello All,

I'm wondering in the SPR phenomena, what is the effective surface area that the light signal is reflected from. For example, if the results is read in terms of SPR signal (association/disscosiation) what is the effective area that the SPR happens at and how much of a modified area is it reading from?
I hope my question is clear.
Thank you

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  • Arnoud
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5 years 11 months ago #2 by Arnoud
Replied by Arnoud on topic Effective surface area of SPR
Hi,

Table: Dimensions and volumes of the Biacore 2000 machine
dimensions (l, w, h)2.4 x 0.5 x 0.05 mm
volume+/- 60 nl
flow cell area1.2 mm2
detector area1.6 x 0.16 = 0.26 mm2 / flow cell

Arnoud

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