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Loss of binding
- Arnoud
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5 years 11 months ago #7
by Arnoud
Replied by Arnoud on topic Loss of binding
Did you check the purity of the free MDMA? Could there be somthing in the prepeartion that is reactive (and destroying) towards the immobilised BSA-MDMA?
If you immobilise the anti MDMA antibody you have an interaction with the BSA-MDMA. Can you block the interaction with the MDMA alone? For instance:
1) anti MDMA (immob) + BSA-MDMA = response
then regenerate
2a) anti MDMA (immob) + MDMA = low response? due to low Mw
no regeneration
2b) inject BSA-MDMA again = no/lower response: MDMA is blocking or same response as in 1 = MDMA is not working
If you immobilise the anti MDMA antibody you have an interaction with the BSA-MDMA. Can you block the interaction with the MDMA alone? For instance:
1) anti MDMA (immob) + BSA-MDMA = response
then regenerate
2a) anti MDMA (immob) + MDMA = low response? due to low Mw
no regeneration
2b) inject BSA-MDMA again = no/lower response: MDMA is blocking or same response as in 1 = MDMA is not working
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