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Anti-Human IgG (Fc) antibody (CM5 immobilization)
- ebelcheva
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6 years 5 months ago #1
by ebelcheva
Anti-Human IgG (Fc) antibody (CM5 immobilization) was created by ebelcheva
Hello,
For the past few years I have been using the an anti-hFc ab from thermo fisher to immobilize on a CM5 chip and perform my capture experiments. The antibody performed very well over multiple experiments and docking in/out biacore system. In my most recent experiments, even right after immobilization, I haven't been able to capture any of my Fc containign proteins with this antibody from Thermo.
I am wondering if anyone has experience with any anti hFc antibody from other vendors than Thermo, for immobilization on CM5 chips. I will be using the human capture kit from GE until I find a less expensive alternative.
Thank you.I would really appreciate your recommendations and suggestions!!
-E
For the past few years I have been using the an anti-hFc ab from thermo fisher to immobilize on a CM5 chip and perform my capture experiments. The antibody performed very well over multiple experiments and docking in/out biacore system. In my most recent experiments, even right after immobilization, I haven't been able to capture any of my Fc containign proteins with this antibody from Thermo.
I am wondering if anyone has experience with any anti hFc antibody from other vendors than Thermo, for immobilization on CM5 chips. I will be using the human capture kit from GE until I find a less expensive alternative.
Thank you.I would really appreciate your recommendations and suggestions!!
-E
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