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KD calculation

  • pcube85
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6 years 5 months ago #1 by pcube85
KD calculation was created by pcube85
How can I determine KD value theoretically. The RU values and time in seconds is given. The analyte concentration is 10 nM. No other information is given.


Thanks,
P.P

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  • Arnoud
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6 years 5 months ago #2 by Arnoud
Replied by Arnoud on topic KD calculation
Hi,

If you have a sensorgram you can fit the association and dissociation phase and calculate the KD.
Look at the section datafitting of the sprpages.

Arnoud

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