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KD calculation
pcube85
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6 years 7 months ago #1
by pcube85
How can I determine KD value theoretically. The RU values and time in seconds is given. The analyte concentration is 10 nM. No other information is given.
Thanks,
P.P
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Arnoud
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6 years 7 months ago #2
by Arnoud
Hi,
If you have a sensorgram you can fit the association and dissociation phase and calculate the KD.
Look at the section datafitting of the sprpages.
Arnoud
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