SPR Guidelines

Dear SPR users

Does any one have information regarding the guidelines to be followed for SPR method validation for binding kinetics. Could you please share.
What parameters can be performed to prove your optimised method is perfect.
I know Precision and Ligand stability other than this any other parameter.

Thanks in advance.

Hi Vini,

You can look into the next publications for more validation parameters.
Depending on your goal more or less validation will be needed.

1. DiGiacomo, R. A., Xie, L., Cullen, C., et al.; Development and validation of a kinetic assay for analysis of anti-human interleukin-5 monoclonal antibody (SCH 55700) and human interleukin-5 interactions using surface plasmon resonance. Analytical Biochemistry (327) 2: 165-175; 2004.
2. Gillis, E., Gosling, J., Sreenan, J., et al.; Development and validation of a biosensor-based immunoassay for progesterone in bovine milk. Journal of Immunological Methods (267) 2: 131; 2002.
3. Gloriam, D. E., Orchard, S., Bertinetti, D., et al.; A Community Standard Format for the Representation of Protein Affinity Reagents. Molecular & Cellular Proteomics (9) 1: 1-10; 2010.
4. Mytych, D., La, S., Barger, T., et al.; The development and validation of a sensitive, dual-flow cell, SPR-based biosensor immunoassay for the detectin, semi-quatification, and chracterization of antibodies tot darbepoetin alfa and epoetin alfa in human serum. 2009.

regards
Arnoud

Hi Around

Thank you very much for the references.
Have you come across any guidelines for SPR.