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Au-S regeneration
- djblevin
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6 years 11 months ago #1
by djblevin
Au-S regeneration was created by djblevin
I am planning on doing an immobilisation/binding assay of glutathione monoester conjugated to a monoclonal antibody, in the hope that the free thiol can interact with a GE Lifescience Au-sensor chip and was wondering what kinds of recovery conditions would I need to remove the immobilised protein of the Au-S interaction.
The SPR machine I use is a BiaCore X100 and the chips are untreated gold sensor chips.
Dankjewel,
djblevin
The SPR machine I use is a BiaCore X100 and the chips are untreated gold sensor chips.
Dankjewel,
djblevin
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- Arnoud
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6 years 11 months ago #2
by Arnoud
Replied by Arnoud on topic Au-S regeneration
Hoi djblevin,
I don't think there is an easy way to remove/break the AU-S interaction.
Please look at
www.researchgate.net/post/Does_sulphur_s...assembled_monolayers
Kind regards
Arnoud
I don't think there is an easy way to remove/break the AU-S interaction.
Please look at
www.researchgate.net/post/Does_sulphur_s...assembled_monolayers
Kind regards
Arnoud
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