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Single Cycle Kinetics
- Biazap
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7 years 7 months ago #1
by Biazap
Single Cycle Kinetics was created by Biazap
Hi Arnoud,
I am seeing a reference flow cell binding when I am trying to do a Single cycle kinetics. I use Biacore T200 and i am trying a capture method.So I immobilise Antihis and capture FcRn. My FC1 has higher bindig than FC2 even for 0 concentration which is the buffer alone.
Please find the attachment for bargraph of FC1.
Need your suggestion.
Thanks
I am seeing a reference flow cell binding when I am trying to do a Single cycle kinetics. I use Biacore T200 and i am trying a capture method.So I immobilise Antihis and capture FcRn. My FC1 has higher bindig than FC2 even for 0 concentration which is the buffer alone.
Please find the attachment for bargraph of FC1.
Need your suggestion.
Thanks
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- Arnoud
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7 years 7 months ago #2
by Arnoud
Replied by Arnoud on topic Single Cycle Kinetics
Hi,
This is strange indeed. I have no direct answer for you. You could try to verify that the injection system is performing alright by doing a system check.
Arnoud
This is strange indeed. I have no direct answer for you. You could try to verify that the injection system is performing alright by doing a system check.
Arnoud
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