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Long spikes before association and after dissociation
- steve123
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7 years 10 months ago #1
by steve123
Long spikes before association and after dissociation was created by steve123
Hi,
I ran my samples from 20-200 uM However, I got two spikes(I can term it peaks)before association and after dissociation. I am not sure whether the fit is good or not. SPR machine details: Reichert Tech.
I ran my samples from 20-200 uM However, I got two spikes(I can term it peaks)before association and after dissociation. I am not sure whether the fit is good or not. SPR machine details: Reichert Tech.
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- Arnoud
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7 years 10 months ago #2
by Arnoud
Replied by Arnoud on topic Long spikes before association and after dissociation
Hi, welcome to the forum.
I think you cannot make a valid fit with these kind of peaks. Probably something went wrong with the injection or data processing.
You can check the injection system with just one injection with buffer. The curves should be flat, if not, clean the system.
I recommend you look at www.sprpages.nl/sensorgram-tutorial/a-sensorgram for an overview of a proper sensorgram shape.
kind regards
Arnoud
I think you cannot make a valid fit with these kind of peaks. Probably something went wrong with the injection or data processing.
You can check the injection system with just one injection with buffer. The curves should be flat, if not, clean the system.
I recommend you look at www.sprpages.nl/sensorgram-tutorial/a-sensorgram for an overview of a proper sensorgram shape.
kind regards
Arnoud
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