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please help
- dan_SPR
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8 years 2 weeks ago #1
by dan_SPR
please help was created by dan_SPR
Hello! I am a new user for BiacoreT200. I did a protein calibration on monday and everydata looks good. But on Tuesday, I did see these signal even with blank buffer injection. I tried desorbing, did not work. Thanks!
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- Arnoud
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8 years 2 weeks ago #2
by Arnoud
Replied by Arnoud on topic please help
Hi Dan,
When injecting the buffer, can you check the tubing behind the left door.
Are they all filled without any air bubbles? Check if all the connections are fastened well.
If the spikes persist, you can check the injection needle if there is some dirt on it. Refer to the maintenance manual how to open the sample compartment.
Kind regards
Arnoud
When injecting the buffer, can you check the tubing behind the left door.
Are they all filled without any air bubbles? Check if all the connections are fastened well.
If the spikes persist, you can check the injection needle if there is some dirt on it. Refer to the maintenance manual how to open the sample compartment.
Kind regards
Arnoud
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- dan_SPR
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8 years 2 weeks ago #3
by dan_SPR
Replied by dan_SPR on topic please help
Hi Arnoud,
I did check the syringe behind the left door. The 500ul one is sooo dirty but not leaky. I have replaced the 500ul syringe and run the desorb again. Will run assay tomorrow morning and let you know the results. Thanks a lot!
Dan
I did check the syringe behind the left door. The 500ul one is sooo dirty but not leaky. I have replaced the 500ul syringe and run the desorb again. Will run assay tomorrow morning and let you know the results. Thanks a lot!
Dan
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