This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.
Streptavidin Chip
- ahmedaboraia
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8 years 10 months ago #1
by ahmedaboraia
Streptavidin Chip was created by ahmedaboraia
Do I need a reference channel in case of using streptavidin chip. And if so what exactly I should use in this reference channel?
Thanks
Thanks
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- Arnoud
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8 years 10 months ago #2
by Arnoud
Replied by Arnoud on topic Streptavidin Chip
Hi Ahmed,
It is better to have a reference channel to compensate for small buffer differences, drift and non-specific binding to the matrix.
The best reference is a related molecule that will not bind the analyte.
When that is not available, use a non related molecule of the same size.
When creating a refrence channel try to match the loading of the ligand with that of the protein of interrest.
For the SA-chip you can think of blocking the streptavidin with biotin to create a 'neutral' surface.
Kind regards
Arnoud
It is better to have a reference channel to compensate for small buffer differences, drift and non-specific binding to the matrix.
The best reference is a related molecule that will not bind the analyte.
When that is not available, use a non related molecule of the same size.
When creating a refrence channel try to match the loading of the ligand with that of the protein of interrest.
For the SA-chip you can think of blocking the streptavidin with biotin to create a 'neutral' surface.
Kind regards
Arnoud
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- ahmedaboraia
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8 years 10 months ago #3
by ahmedaboraia
Replied by ahmedaboraia on topic Streptavidin Chip
Thanks a lot Arnoud
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