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Double peak
- avsurround
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8 years 9 months ago #1
by avsurround
Double peak was created by avsurround
Hello,
I've been doing some fragment library screening using 12.5k RU of immobilized protein and 1 mM fragments in 2% DMSO. The majority of sensorgrams are looking quite good except for some that exhibit a double peak. Where could the problem be?
Too much protein? Too high concentration of analyte? Fragment-structure dependent? Flow rate not fast enough?
Thanks!
I've been doing some fragment library screening using 12.5k RU of immobilized protein and 1 mM fragments in 2% DMSO. The majority of sensorgrams are looking quite good except for some that exhibit a double peak. Where could the problem be?
Too much protein? Too high concentration of analyte? Fragment-structure dependent? Flow rate not fast enough?
Thanks!
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- Arnoud
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8 years 9 months ago #2
by Arnoud
Replied by Arnoud on topic Double peak
Hi,
Speculation: maybe the mixing of the analyte in the vial? Possible aggregation of analyte?
Or just bad luck.
Kind regards
Arnoud
Speculation: maybe the mixing of the analyte in the vial? Possible aggregation of analyte?
Or just bad luck.
Kind regards
Arnoud
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