Hİ everybody,
I use Bionavi210A SPR. I'm still learning. I try to immobilze HSA with amino coupling on CMD (=CM5) chip. But my protein signal is very low. My coupling buffer is 10 mM Na Acetate pH: 5.2. HSA is globulin and fatty acid free. I tried a lot of different chemical such as different protein (BSA), different EDC and NHS, Different buffer ( 5 mM MES pH: 5.0) but nothing changes. I also tried different concentraion of proteins. I sent you my sensogram results.
I sent you my sensogram results for BSA and HSA .
Do you have any recommadation to solve this problem?