To detect (100 RU Rmax) a 200 Da molecule with your 66kDa protein you need approximately 33000 RU of 66 kDA protein on the chip. To capture 33000 RU of protein with an antibody, you need about 37500 RU of antibody that can capture two proteins. Of course, this is all theoretically.
When you go for less signal (its better), like 20 RU, the amount of antibody to immobilize is less (7500 RU), which is not too difficult to do.
Use a high capacity sensor chip, like the Biacore CM7, Bio-Rad GLH or Xantec CMD500D.
Or try it first with a normal one.
Let us know if it worked out for you!
Arnoud