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buffer with EDTA
- papillon
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10 years 9 months ago #1
by papillon
buffer with EDTA was created by papillon
Hi,
I would like to prepare a buffer with EDTA and it´s the first time I would like to know whta EDTA I should use. EDTA sodium salt? EDTA sodium dihidrate?
Thanks everybody
Papillon
I would like to prepare a buffer with EDTA and it´s the first time I would like to know whta EDTA I should use. EDTA sodium salt? EDTA sodium dihidrate?
Thanks everybody
Papillon
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- Arnoud
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10 years 9 months ago #2
by Arnoud
Replied by Arnoud on topic buffer with EDTA
Hi Pappillon,
Ethylenediaminetetra acetic acid disodium salt dihydrate: Mw 372.24 is OK.
Make a 0.5 M solution by dissolving in pure water and adding NaOH until pH 8.0.
EDTA dissolves readily at pH 8.0 but very slowly at pH < 8.0.
Sterilise by autoclaving or 0.22 um filter. You can store it for a long time (> 1 year).
Arnoud
Ethylenediaminetetra acetic acid disodium salt dihydrate: Mw 372.24 is OK.
Make a 0.5 M solution by dissolving in pure water and adding NaOH until pH 8.0.
EDTA dissolves readily at pH 8.0 but very slowly at pH < 8.0.
Sterilise by autoclaving or 0.22 um filter. You can store it for a long time (> 1 year).
Arnoud
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