This forum is intended for questions about kinetics, Surface Plasmon Resonance and the instruments related to these techniques.
immobilization/ amine coupling
- Cotix
- Topic Author
- Visitor
-
10 years 10 months ago #1
by Cotix
immobilization/ amine coupling was created by Cotix
Hi,
I would like to immobilize a basic protein (Theoretical pI: 9,53) by amine coupling to sensor CM5 or CM4. I am afraid that immobilization in recommended conditions (pH 6.0) will lead to inactivation of the protein. What do you think about amine coupling in more physiological conditions (about pH 7.4)?
I wouldn’t like to use SA sensor.
Thank you.
Cotix
I would like to immobilize a basic protein (Theoretical pI: 9,53) by amine coupling to sensor CM5 or CM4. I am afraid that immobilization in recommended conditions (pH 6.0) will lead to inactivation of the protein. What do you think about amine coupling in more physiological conditions (about pH 7.4)?
I wouldn’t like to use SA sensor.
Thank you.
Cotix
Please Log in or Create an account to join the conversation.
Moderators: Arnoud