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Inverted curve
- OldForum
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12 years 8 months ago #1
by OldForum
Inverted curve was created by OldForum
I ran a TSH sample using HBS P+ with 0.5M NaCL. Back in 6/15/11 I got some binding on the Capture level for the Ab. Then 8/15/11, I ran the same system and now I am getting an inverted curve. I wanted to make sure I did not grab the wrong diluent so I repeated the test ensuring that the same buffer for the assay is used in diluting the samples. It still showed an inverted peak. Any ideas on what maybe going wrong?
mtarca
mtarca
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- OldForum
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12 years 8 months ago #2
by OldForum
Replied by OldForum on topic Inverted curve
Hi,
Can you tell how much down your curves go? Is it a curve or more a buffer jump?
What happens when you inject buffer alone? Check the injection system.
Arnoud
Can you tell how much down your curves go? Is it a curve or more a buffer jump?
What happens when you inject buffer alone? Check the injection system.
Arnoud
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- OldForum
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12 years 8 months ago #3
by OldForum
Replied by OldForum on topic Inverted curve
Here are sensorgrams of the the analysis..the flat line is the buffer. Top one was in 6-15. Bottom one was in 8-16. One thing did change though in-between these times is a new IFC was installed. But did not think this was going to make a difference.
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