These are the posts of the old forum. It was not possible to transfer the user data, so they are missing in most of the posts. For new questions, go to the general discussions.

Chip preconditioning

OldForum
Topic Author
Visitor

13 years 7 months ago #1 by OldForum

Chip preconditioning was created by OldForum

Is it necessary to run these injections (sodium hydroxide, hydrochloric acid, SDS and/or phosphoric acid) to precondition a C1 chip? Or is it just necessary/recommended for dextran chips?

Thanks,
joseantonio

Please Log in or Create an account to join the conversation.

OldForum
Topic Author
Visitor

13 years 7 months ago #2 by OldForum

Replied by OldForum on topic Chip preconditioning

i've just open a c1 chip, they say to injected with a solution prepared with 0.1M glycine-NaOH pH 12 with 0.3% triton buffer.! then do a prime with the running buffer

cheers
pierqiuseppe

Please Log in or Create an account to join the conversation.

OldForum
Topic Author
Visitor

13 years 7 months ago #3 by OldForum

Replied by OldForum on topic Chip preconditioning

Thanks. I am using my own chips, but they are very like a C1 chip. I will include that injections in my routines. BTW, I build my SAMs with MUA, but does anybody knows how they prepare the C1 chips exactly? It is just curiosity.
joseantonio

Please Log in or Create an account to join the conversation.

SPR-Pages

Chip preconditioning