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DNA-SH immobilisation
- OldForum
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13 years 7 months ago #1
by OldForum
DNA-SH immobilisation was created by OldForum
could anyone indicatea protocol for DNA-SH immo onto gold surface ?
thanks
dd
thanks
dd
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- OldForum
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13 years 7 months ago #2
by OldForum
Replied by OldForum on topic DNA-SH immobilisation
Why would you want to immobilize the SH-DNA directly to the gold surface?
Ik think that using a dextran coated chip and a thiol immobilizing protocol will be more efficient.
Arnoud
Ik think that using a dextran coated chip and a thiol immobilizing protocol will be more efficient.
Arnoud
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- OldForum
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13 years 7 months ago #3
by OldForum
Replied by OldForum on topic DNA-SH immobilisation
Just dilute the thiolated DNA (~1uM) in a buffer (I use PBS) and incubate it over a couple of hours. Then, if you want, do an extra half hour long incubation of MCH (1mM) in the dark.
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