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small molecule immobilisation
- OldForum
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17 years 2 months ago #1
by OldForum
small molecule immobilisation was created by OldForum
Hi!
I`m trying to immobilize a MW 500 molecule(NHS active ester) on a modified (bearing free amine groups) sensor surface. Has anyone experience in this field?
I would be deeply grateful to any reply concerning this topic.
Pharmchem
I`m trying to immobilize a MW 500 molecule(NHS active ester) on a modified (bearing free amine groups) sensor surface. Has anyone experience in this field?
I would be deeply grateful to any reply concerning this topic.
Pharmchem
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- OldForum
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17 years 2 months ago #2
by OldForum
Replied by OldForum on topic small molecule immobilisation
Hi,
I did it once the other way round. Attach a NH2 bearing small molecule to the activated ester on the chip. A 1 mM concentration of the small molecule (check solubility!) worked fine in a HBS-N buffer system at 5ul/min with a 10-20 min. injection. The immobilization level was generally in the range of 100-200 RU. Depending on the later experiment u might have to reduce the injection time to reduce the surface capacity. I guess it should work in your set-up as well.
greetz
Strada
I did it once the other way round. Attach a NH2 bearing small molecule to the activated ester on the chip. A 1 mM concentration of the small molecule (check solubility!) worked fine in a HBS-N buffer system at 5ul/min with a 10-20 min. injection. The immobilization level was generally in the range of 100-200 RU. Depending on the later experiment u might have to reduce the injection time to reduce the surface capacity. I guess it should work in your set-up as well.
greetz
Strada
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