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Linked reactions
- OldForum
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19 years 5 months ago #1
by OldForum
Linked reactions was created by OldForum
Hi all,
I am trying to identify a conformational change upon Analyte binding to Ligand. I know that different dissociation rates with different injecting times at a constant analyte concentration is the method to resolve this question. But the A-L interaction does not reach steady-state even at high analyte concentrations (200 nM) even at long injection times.
Is there any solution?
thanks in advance and good luck with your experiments
I am trying to identify a conformational change upon Analyte binding to Ligand. I know that different dissociation rates with different injecting times at a constant analyte concentration is the method to resolve this question. But the A-L interaction does not reach steady-state even at high analyte concentrations (200 nM) even at long injection times.
Is there any solution?
thanks in advance and good luck with your experiments
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- OldForum
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19 years 5 months ago #2
by OldForum
Replied by OldForum on topic Linked reactions
Try to calculate if your goal is feasable.
You can calculate the Req
Req = (ka . C / ka . C + kd) Rmax
You can estimate the time to reach equilibrium
teq = -ln(1-@)/(ka . C + kd)
where teq is the time to equilibrium and @ is the fraction of reached equilibrium, typical 95% thus 0.95
Arnoud
You can calculate the Req
Req = (ka . C / ka . C + kd) Rmax
You can estimate the time to reach equilibrium
teq = -ln(1-@)/(ka . C + kd)
where teq is the time to equilibrium and @ is the fraction of reached equilibrium, typical 95% thus 0.95
Arnoud
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