Is the equilibrium dissociation constant all what matters?
In my opinion, reporting a single value for a kinetic interaction is hiding all the other information present in the measurements between two or more components.
Improving protein quality
Is your protein affected by the buffer and immobilization conditions used? Your assay problems might be caused by poor protein quality!
Luckily there is an easy and fast way to check the integrity of your proteins. Nanotemper Technologies provides various instruments to check your protein quality, all using a minimum of sample. .
SPR is not an Art!
Blog of Ryan Denomme
Ryan Denomme of Nicoya publishes a nice blog about SPR and related topics. For instance about preserving precious sample by optimizing preconcentration and immobilization or what the difference is between SPR and LSPR.
Definitely worthwhile to check this blog!
How Low Can You Go ?
This depends in the first place on the instrument you have chosen. The signal should be above the noise level of the baseline. The curves should be easily recognisable and replicates should overlay.
25 Years Of SPR
The instruments are all in best shape and performing excellent. However, how is the user doing? Has he also developed with the instruments or is he struggling to keep up?