Removing ligand

When taking the chip out of the casing, avoid contact with either chip surfaces. Put in a 50 ml tube and do all the incubations mentioned in the article.
After washing, first dry all of the holder except the chip.
The use a clean tissue like Kimwipes:

Lokate the chip surface which is level with the holder. This is the sensor side. Clean dry and dust free.
Lokate the other side (indented) which is the surface with the dextran. Put the Kimpwipe in a corner letting the excess liquid flow in it. Do not wipe this surface.

Insert the holder in the casing (there is only one way which goes easy) and your chip is regnerated.

Thanks for your reply. i will try your suggestions. However i still think it could be easier to inject 60ul of Pronase onto the chip and then undock the chip and leave it overnight. TheBIacore machine could easily be cleaned by using NaOH. However I cannot work out how to inject a solution onto the chip and then leave it there. I suspect that BIAcore has designed the software so that people cannot do this as it would be easier to regenerate the chip this way. I mainly want to use the renerated chip as a screening chip. When I have my buffer conditions correct i would then use a new chip.

Thankyou for your suggestions